| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217264 |
| Chromosome: | chromosome 3 |
| Location: | 6417257 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCGTGTAGAACGCAGCGGGACCGGGA |
| Internal bar code: | AAAGGCTCCTCCGATGCCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 99.5251 |
| LEAP-Seq n confirming: | 3982 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGGCTAGGACTCACCAG |
| Suggested primer 2: | GTAGAAACCGCCCAGTGTGT |