| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217264 |
| Chromosome: | chromosome 6 |
| Location: | 4944712 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279650 | OPR24 | OctotricoPeptide Repeat protein 24 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCCATCGCGCCCTCCCCGCTGCCCGCC |
| Internal bar code: | AAGCGATGTGCTACTTACTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 24 |
| LEAP-Seq percent confirming: | 98.9691 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGGAGCTGTTGTCCTG |
| Suggested primer 2: | GCTCAGGCTGAAAACCTGTC |