| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217278 |
| Chromosome: | chromosome 7 |
| Location: | 3574212 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCGCACTCCCAGGAACCTGCTGTGCT |
| Internal bar code: | GATTATTGGAAGCTTTCGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1172 |
| LEAP-Seq percent confirming: | 89.011 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTTATTTGCTGCCTTAC |
| Suggested primer 2: | AACTAACAACGATCACCGGC |