Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.217295 |
Chromosome: | chromosome_16 |
Location: | 4899583 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g685750 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCGGCGCCGCCCGCTTACGTCCCCGTGGT |
Internal bar code: | GCGGGTCGTGCCCTTCGTGCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 119 |
LEAP-Seq percent confirming: | 98.8208 |
LEAP-Seq n confirming: | 419 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGTGGAGAAGGTCAGAGC |
Suggested primer 2: | CGAGTCACCAACCACATGAC |