| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217299 |
| Chromosome: | chromosome 11 |
| Location: | 3282578 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTAACGAAATGCGAATGCAAAATCGACC |
| Internal bar code: | AACGGCGGGCACAATCGGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 99.1475 |
| LEAP-Seq n confirming: | 5815 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCACTGTCGACCTATGC |
| Suggested primer 2: | ATCCAAGGAAATACCCCCAG |