| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217318 |
| Chromosome: | chromosome 13 |
| Location: | 3876911 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590300 | URK2,CMPK1 | (1 of 1) PTHR10285:SF76 - URIDINE KINASE; Uridine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGAATAATGCCAGAGACGTGCGGTCAT |
| Internal bar code: | TTGTTTTTCAGGTGGCAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 96.695 |
| LEAP-Seq n confirming: | 4213 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGACTTTCAAGCGCGGATT |
| Suggested primer 2: | TTTAACTCCCCCAACAGCAC |