| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217352 |
| Chromosome: | chromosome_13 |
| Location: | 3594926 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g588350 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACGGTGCCCCGCAGAATGCCGTGCCCAAG |
| Internal bar code: | GGTAACCTACCGCAAAAGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 96.3068 |
| LEAP-Seq n confirming: | 339 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACATGCCAACCAGTC |
| Suggested primer 2: | CCCATCGCAAGTTAAACGAT |