| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217357 |
| Chromosome: | chromosome 7 |
| Location: | 2250182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327450 | DNJ34 | DnaJ-like protein; (1 of 1) PTHR24078//PTHR24078:SF201 - DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTAGCATGTGGGCCCGTGCGTGGAGGA |
| Internal bar code: | CTTCGTTGGCCATCACGATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 99.143 |
| LEAP-Seq n confirming: | 14460 |
| LEAP-Seq n nonconfirming: | 125 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGTCACTAGGGGTGCAT |
| Suggested primer 2: | GGACGATTTCGTTGCGTATT |