| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217363 |
| Chromosome: | chromosome 5 |
| Location: | 3203839 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239800 | ARS14 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGTGTCGCTGTGGAGGGGCGGTGGGG |
| Internal bar code: | TCGGTGGCATTTAATATCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 94.8415 |
| LEAP-Seq n confirming: | 7777 |
| LEAP-Seq n nonconfirming: | 423 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGTACTTCAGGGCTGC |
| Suggested primer 2: | GGGTAGGCAAATGACAGCAT |