| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217401 |
| Chromosome: | chromosome 12 |
| Location: | 8110215 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551501 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATCTGTATGTAGCTTTTGTGTTCCGTCA |
| Internal bar code: | GCTCGGAACAGCGTAGGCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 99.7004 |
| LEAP-Seq n confirming: | 4659 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACTCAGCTTCCGTCGAA |
| Suggested primer 2: | TAGTTATGCCGTGCCCCTAC |