Insertion junction: LMJ.RY0402.217408_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.217408
Chromosome:chromosome 2
Location:2512126
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g092250 FKB42,FKB22 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 2) PF00254//PF07719//PF13414 - FKBP-type peptidyl-prolyl cis-trans isomerase (FKBP_C) // Tetratricopeptide repeat (TPR_2) // TPR repeat (TPR_11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGTAGTGACGTGGAAAGGGCAGGGGCAT
Internal bar code:ATCTAACAGTATCACTTAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:992
LEAP-Seq percent confirming:50.8333
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAAACCAAGCCAAACAAG
Suggested primer 2:GTAGATGGCCGTGTTCCAGT