| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217443 |
| Chromosome: | chromosome 7 |
| Location: | 5284426 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349300 | HEL41 | (1 of 2) PTHR24031//PTHR24031:SF303 - RNA HELICASE // DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL; DEAD box ATP-dependent RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCCAACAAGGCCGCGCGGGGGCCGCGG |
| Internal bar code: | GCCGACGGGGGGTTCCTGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 99.7618 |
| LEAP-Seq n confirming: | 2513 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGCTATGGGGTTGTGGG |
| Suggested primer 2: | CGCCTAGGAGCTATCACTGG |