Insertion junction: LMJ.RY0402.217461_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.217461
Chromosome:chromosome_13
Location:3464842
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g587550 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GGTGTGTGTAAGATGGGAAGCGGGCCCACA
Internal bar code:CTGCAGATGCGGGTATCGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1054
LEAP-Seq percent confirming:98.3029
LEAP-Seq n confirming:18072
LEAP-Seq n nonconfirming:312
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTACTGTTTGCTTGAGGC
Suggested primer 2:GCAATGAGCCAGACATGAGA