| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217463 |
| Chromosome: | chromosome 4 |
| Location: | 2287128 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220350 | ATPVA3 | Vacuolar ATP synthase subunit A; (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGCGTCGAGATCTGCGTCGAGCTCTGC |
| Internal bar code: | GGGTGGCGACGCACGTAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 97.3292 |
| LEAP-Seq n confirming: | 7835 |
| LEAP-Seq n nonconfirming: | 215 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCATTCTCAAAAGCCCTG |
| Suggested primer 2: | CAGAGATTGGGAGATTGGGA |