Insertion junction: LMJ.RY0402.217481_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.217481
Chromosome:chromosome 10
Location:1472913
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g428692 (1 of 1) IPR000945//IPR002110//IPR020683 - Dopamine beta-hydroxylase-related // Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAAACAAGCGCTGGGCGGCAAAACGTTG
Internal bar code:ATTACCGGATTGTCCGCGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:33
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAACGACAGGGATGTTGG
Suggested primer 2:GAGGTAGCTCTCGAACACGG