Insertion junction: LMJ.RY0402.217500_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.217500
Chromosome:chromosome 4
Location:1239080
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g215250 FAP26 Ankyrin Repeat Flagellar Associated Protein 26; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAGAAAGTTTTGATAGCATACCTCTTG
Internal bar code:CGCCCAACGTGACGGCTGTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:740
LEAP-Seq percent confirming:99.7378
LEAP-Seq n confirming:6846
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTGGGTATGACATCGTG
Suggested primer 2:GCTGCAGACGTGATAGTCCA