| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217520 |
| Chromosome: | chromosome 11 |
| Location: | 461998 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467591 | (1 of 1) K12840 - splicing factor 45 (RBM17, SPF45) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTGAAGATGAGGAGGCGGGGCAGATGT |
| Internal bar code: | ACCGGGCAACAAATTCATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 99.7241 |
| LEAP-Seq n confirming: | 723 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGGTGCTAGGGGTTGT |
| Suggested primer 2: | TGCGCATCTTTGTTCAGTTC |