| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217520 |
| Chromosome: | scaffold 19 |
| Location: | 81689 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750597 | CDPK2 | Calcium/calmodulin-dependent protein kinase; (1 of 2) PF00069//PF13499 - Protein kinase domain (Pkinase) // EF-hand domain pair (EF-hand_7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCCGTAGATGAGCACCGCTAAGCACTC |
| Internal bar code: | ACTGCTAGTGCTATGCCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 776 |
| LEAP-Seq percent confirming: | 99.5244 |
| LEAP-Seq n confirming: | 5022 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCGTTAACCCAGTACCC |
| Suggested primer 2: | TACACCCACTCACCCCTCTC |