| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217524 |
| Chromosome: | chromosome_10 |
| Location: | 4249664 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g450350 | IFT25,FAP232 | Intraflagellar transport protein 25 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTTTACAGATGCAGCACCTTGACAGGGCC |
| Internal bar code: | GGAGTTGTCCCGACTGAGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 56 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 176 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACAGTAGGGGCCACATT |
| Suggested primer 2: | ATTTTGCATGGCTTGAGGTC |