Insertion junction: LMJ.RY0402.217527_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.217527
Chromosome:chromosome 15
Location:133195
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g634950 Protein tyrosine kinase; (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAAGCCGTACCCGGCACCAGTGCCGCCG
Internal bar code:TGCTGTGCGAGGTGCAACGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:34
LEAP-Seq percent confirming:0.662043
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:3151
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCACAAAGCACCACCACA
Suggested primer 2:CATGAATGTAACCCTCCCCA