Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.217562 |
Chromosome: | chromosome_8 |
Location: | 966077 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g361950 | FAL10 | Similar to Flagellar Associated Protein FAP72 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGCCACGCGCCAGGACACGCCGGGGAACT |
Internal bar code: | GCATCCGACTTTGAATAGATTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 181 |
LEAP-Seq percent confirming: | 99.8031 |
LEAP-Seq n confirming: | 507 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAAGCGAGGGACTGGAGAGA |
Suggested primer 2: | CCTTGAGTGCGCTCTTCTTT |