Insertion junction: LMJ.RY0402.217562_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.217562
Chromosome:chromosome 8
Location:966077
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g361950 FAL10,FAP153 Flagellar Associated Membrane Protein 153 with PAS Sensory Domain; (1 of 28) PTHR31600//PTHR31600:SF2 - FAMILY NOT NAMED // TINY MACROCYSTS PROTEIN B-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCCACGCGCCAGGACACGCCGGGGAACT
Internal bar code:GCATCCGACTTTGAATAGATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:181
LEAP-Seq percent confirming:99.8031
LEAP-Seq n confirming:507
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGCGAGGGACTGGAGAGA
Suggested primer 2:CCTTGAGTGCGCTCTTCTTT