| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217593 |
| Chromosome: | chromosome 2 |
| Location: | 4300366 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099055 | (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGTAAGCAAACAAAGTTGCAAGAAGTG |
| Internal bar code: | TATCCCCACACGCGACGTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 99.8104 |
| LEAP-Seq n confirming: | 7371 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTTGGTCGGTTGTCTCC |
| Suggested primer 2: | GTCATCCACTTTGACGCCTT |