| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217600 |
| Chromosome: | chromosome 6 |
| Location: | 3796944 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278164 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTGTCAGTGGCGCCCATGGCGGGCAG |
| Internal bar code: | CCGAAGAGTGCGTGCAAAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 97.3621 |
| LEAP-Seq n confirming: | 1218 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGTCTGGCTCTTGCTCC |
| Suggested primer 2: | GTCAACCGTTTTCCACGAGT |