| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217652 |
| Chromosome: | chromosome 1 |
| Location: | 2904657 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017650 | FAP34,RIB30 | Ribbon binding 30 kDa microtuble inner protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCCGTGCCTGGATTGCCCCCGCCCCCG |
| Internal bar code: | GCGGTTGGGTCACAGTCAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.6905 |
| LEAP-Seq n confirming: | 20934 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACTCCTTTGTGGGGAAA |
| Suggested primer 2: | GAAGAGCATGGCCTGTAAGC |