| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217666 |
| Chromosome: | chromosome 9 |
| Location: | 5515404 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400478 | DIV31 | phosphatidylinositol 4-phosphate 5-kinase; (1 of 1) PTHR23086:SF8 - PROTEIN PPK-2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGGCAAGTGCCGGCCTGGCCTCGAGCA |
| Internal bar code: | TTGGGATCAAGACAATCTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 623 |
| LEAP-Seq percent confirming: | 99.044 |
| LEAP-Seq n confirming: | 7252 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCTCCTCCTCCTTCTC |
| Suggested primer 2: | TTCTACCGCCAACAATTTCC |