| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217680 |
| Chromosome: | chromosome 10 |
| Location: | 3128767 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441850 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGGGTCGGTTCGCACCTTGCGACAGTC |
| Internal bar code: | GTTTTAGAACTCAGGCTGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1162 |
| LEAP-Seq percent confirming: | 95.6835 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTTGCAGGTAGGCAAGC |
| Suggested primer 2: | GACCTGGACAGCCTTCTCAC |