Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.217680 |
Chromosome: | chromosome 11 |
Location: | 2192057 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCACCAGCCTGTGAGAGTGCGCGTGCG |
Internal bar code: | ACAGCCTTAAAGCGGGTCGCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 731 |
LEAP-Seq percent confirming: | 67.8974 |
LEAP-Seq n confirming: | 1085 |
LEAP-Seq n nonconfirming: | 513 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGCACCATGTACTCCACAA |
Suggested primer 2: | TCCAGCGTTCGCTTTAGTTT |