| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217680 |
| Chromosome: | chromosome 13 |
| Location: | 957793 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568200 | AXL1 | Arabinose chain extension enzyme like protein 1; (1 of 3) PTHR10994//PTHR10994:SF69 - RETICULON // PROLINE-RICH PROTEIN 18 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAGGTGGCGTAGTCCCGTAGTGTGCAG |
| Internal bar code: | TGTTGGTGAACCTCGTCTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 99.5526 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTCTTTTGAGGTGGGGA |
| Suggested primer 2: | GCTGTAACACGGCCCATACT |