Insertion junction: LMJ.RY0402.217688_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.217688
Chromosome:chromosome 9
Location:3870114
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g391986 (1 of 1) 3.1.1.32 - Phospholipase A(1) / Phospholipase A1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCATGGGTCAATCGAACGGACAACCAAC
Internal bar code:AGACTGGGCGGTCGACATGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:787
LEAP-Seq percent confirming:99.7886
LEAP-Seq n confirming:4721
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACAGCACCAAAACACAT
Suggested primer 2:AATGCGGATGTTACAGAGGG