| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217702 |
| Chromosome: | scaffold 19 |
| Location: | 35725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750397 | (1 of 1) 3.2.1.17 - Lysozyme / Muramidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCGATGGGACAGGGTTGAGGCAGGTTG |
| Internal bar code: | CCCTACGTCTAGCTCATATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.023 |
| LEAP-Seq n confirming: | 7399 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTACCGTAGG |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |