| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217704 |
| Chromosome: | chromosome 16 |
| Location: | 1151574 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650350 | (1 of 1) PF03457 - Helicase associated domain (HA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGGTGGGTGGAGATGTTCCCACCGTGA |
| Internal bar code: | ACTGGCGTACCGCGGCTGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 98.4369 |
| LEAP-Seq n confirming: | 15177 |
| LEAP-Seq n nonconfirming: | 241 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCCATACGTCACCACAA |
| Suggested primer 2: | CCTTTCTCCACATCAACGGT |