| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217726 |
| Chromosome: | chromosome 1 |
| Location: | 4811833 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033300 | (1 of 1) PF13668 - Ferritin-like domain (Ferritin_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCTTGCTGCGATTGCTTTTGCGGCTGC |
| Internal bar code: | ACGCACTGTGGCAAGTCATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 99.5455 |
| LEAP-Seq n confirming: | 1095 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATCAATTCTGCGTGCTG |
| Suggested primer 2: | CTAAGACCGCGTGAAGAAGG |