| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217730 |
| Chromosome: | chromosome 16 |
| Location: | 113433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695400 | (1 of 2) PF09733 - VEFS-Box of polycomb protein (VEFS-Box) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTCTCACAAGCTCGCAAGCTCCAACGT |
| Internal bar code: | ACGCGCAAGTAGTCGAGATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 783 |
| LEAP-Seq percent confirming: | 98.998 |
| LEAP-Seq n confirming: | 1482 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTAGGCGCGATTCCTGTA |
| Suggested primer 2: | CCATCCAATCCCAACATACC |