Insertion junction: LMJ.RY0402.217733_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.217733
Chromosome:chromosome 1
Location:4634703
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g031900 (1 of 3) PTHR10566//PTHR10566:SF76 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // PROTEIN Y32H12A.7 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTCGAACCACATACACAGTGCTCTAGG
Internal bar code:AAGCCGCCTTGAGGCAACGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:608
LEAP-Seq percent confirming:76.1185
LEAP-Seq n confirming:4883
LEAP-Seq n nonconfirming:1532
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GATCCAACCTGCACCAACTT
Suggested primer 2:TGGACATGTCTGCTAGGCTG