Insertion junction: LMJ.RY0402.217745_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.217745
Chromosome:chromosome 9
Location:3342437
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g388504 (1 of 16) IPR003903 - Ubiquitin interacting motif CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGGCCACGTCCGCCTGCCAGGCTGCGA
Internal bar code:AGGGGCTGGGAACGGGCAGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:383
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:134
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATTACCTTGTCACGCCTTGG
Suggested primer 2:GAGAGTGGTAGCGGCTTACG