| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217746 |
| Chromosome: | chromosome 2 |
| Location: | 6422382 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115600 | MSC7 | Predicted protein with mechanosensitive ion channel domain; (1 of 1) IPR002048//IPR006685//IPR010920//IPR020568 - EF-hand domain // Mechanosensitive ion channel MscS // LSM domain // Ribosomal protein S5 domain 2-type fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCTCGTCAAACGTCAACAGGCCTGCGC |
| Internal bar code: | ACGGTACCACATCGGCAAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 98.511 |
| LEAP-Seq n confirming: | 1257 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGGACGAGGCAACTAGG |
| Suggested primer 2: | ACTGCACCAGCTACGGCTAC |