| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217747 |
| Chromosome: | chromosome 6 |
| Location: | 3771612 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278159 | CRCO,CO,CON1,CONSTANS | CONSTANS-like protein involved in circadian rhythms; (1 of 1) PF00643//PF06203 - B-box zinc finger (zf-B_box) // CCT motif (CCT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCTCGAGAGAGAACAAAGCCTGCCCCC |
| Internal bar code: | ACCGGTTGGAACGGGGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 98.7578 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTGCTGGAAGTACTGGA |
| Suggested primer 2: | GCAATGAGACAGTCAAGGCA |