Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.217749 |
Chromosome: | chromosome 12 |
Location: | 3516430 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g513100 | (1 of 1) 3.2.1.165 - Exo-1,4-beta-D-glucosaminidase / GlcNase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGTCTGTACGCTGTACGCTGTACCAATT |
Internal bar code: | GCACAGGCAACCGCTGTGTCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 354 |
LEAP-Seq percent confirming: | 99.9168 |
LEAP-Seq n confirming: | 2401 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGACTCCCTGTGTTGCAAAT |
Suggested primer 2: | CTACACGCCCTCCTACAACC |