| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217752 |
| Chromosome: | chromosome 6 |
| Location: | 7965587 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303650 | (1 of 1) K15027 - translation initiation factor 2D (EIF2D) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAACAGTTGTGACACAAGCACCAGCCCG |
| Internal bar code: | GGGTAGCAAACAACGAGGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.2762 |
| LEAP-Seq n confirming: | 8504 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGGAAGGGTGTCACTG |
| Suggested primer 2: | GCTGAATCCTGCTCATGTCA |