| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217755 |
| Chromosome: | chromosome 14 |
| Location: | 3215464 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629150 | CYG4 | Adenylate/guanylate cyclase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGCCAGTCACTGACCTGCAAACACGTC |
| Internal bar code: | TATGCTCCCGCCGCGATGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1028 |
| LEAP-Seq percent confirming: | 99.889 |
| LEAP-Seq n confirming: | 3600 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCAGGAACGGCTCGACTA |
| Suggested primer 2: | ACTCACGCCCATACCAAAAG |