| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217758 |
| Chromosome: | chromosome_10 |
| Location: | 91994 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g418250 | BLD10 | Basal body protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTACATAGGAACGACCAGGAATGTTGAAGG |
| Internal bar code: | GAAACGGAGCAGATGTGAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 96.056 |
| LEAP-Seq n confirming: | 1510 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTATCATGGGCTGGAATA |
| Suggested primer 2: | ATGATCTGGTGGGTAGGCTG |