Insertion junction: LMJ.RY0402.217777_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.217777
Chromosome:chromosome_3
Location:4759625
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g178100 Lipoxygenase sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCGAGCGCAACCGCGAGGACGTGTTCACG
Internal bar code:TGCCCCCAGCGGGGAGAAGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:694
LEAP-Seq percent confirming:99.799
LEAP-Seq n confirming:4964
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GGGACAGGTTGACACGTTCT
Suggested primer 2:GCTCTCACAACCCAACGACT