| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217784 |
| Chromosome: | chromosome_10 |
| Location: | 4166434 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g449750 | PKS1 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCTGGGCGTGCTACAGCCGCCTGCGCCGG |
| Internal bar code: | GGCACCAGAGCGGCCGTGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 91.4302 |
| LEAP-Seq n confirming: | 1643 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTAGCAGTAGCAGCAGCG |
| Suggested primer 2: | CATTGTACATCGAGGCAAGC |