| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217804 |
| Chromosome: | chromosome 11 |
| Location: | 3169503 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479350 | (1 of 3) PTHR31563//PTHR31563:SF1 - FAMILY NOT NAMED // ION CHANNEL POLLUX-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGCCTGTGCAAGGTGCACCCGGCTTT |
| Internal bar code: | GTTGGGCGGAGGCTTGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 99.6696 |
| LEAP-Seq n confirming: | 5732 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAAAGCAAGAAGGAAGC |
| Suggested primer 2: | GTGGAGCCACTGGTATTGCT |