| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217859 |
| Chromosome: | chromosome 14 |
| Location: | 1732217 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619613 | (1 of 1) PF04855 - SNF5 / SMARCB1 / INI1 (SNF5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTCAACCTTGCAACCCGTTCCCCCCCG |
| Internal bar code: | TACTGACTGGTACAGTTGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 93.2819 |
| LEAP-Seq n confirming: | 847 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTCTCCTCCTGTTCGTG |
| Suggested primer 2: | CTAAACCCGAAATGCAGGAA |