| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217864 |
| Chromosome: | chromosome 16 |
| Location: | 5635748 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679550 | FAP277 | Flagellar Associated Protein 277; (1 of 1) K13963 - serpin B (SERPINB) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGGGGTTCTGGACGCACGCCTTCAAG |
| Internal bar code: | GCGGACTGCATGCGGGAGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 99.7587 |
| LEAP-Seq n confirming: | 11160 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGTTCGTCATCCCCTCA |
| Suggested primer 2: | TTCTTAGCCCCATGAGCATC |