| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217867 |
| Chromosome: | chromosome 14 |
| Location: | 2813632 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627411 | (1 of 2) PTHR13146//PTHR13146:SF0 - UNCHARACTERIZED // SOLUTE CARRIER FAMILY 35 MEMBER F6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCAGGTGACTAACTGCGAGATGGAGGA |
| Internal bar code: | TACTTGTTATTTTGTATGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 98.9114 |
| LEAP-Seq n confirming: | 1999 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGACAGCAGTCCAAACCC |
| Suggested primer 2: | TCATATGGATTGCTTGGTGC |