| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217897 |
| Chromosome: | chromosome 2 |
| Location: | 1964659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088100 | (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGGGTTACATGGCCTATGACGGGACTG |
| Internal bar code: | TGCCATGGTCCCAGAGAATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1072 |
| LEAP-Seq percent confirming: | 99.6866 |
| LEAP-Seq n confirming: | 4135 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTCATTGTATGTCAACC |
| Suggested primer 2: | TTCTTTCTCGCTGGGTGAGT |