| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217898 |
| Chromosome: | chromosome_10 |
| Location: | 1287451 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g427300 | RSP2 | Radial spoke protein 2 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGAAAGAATAGCTGTGTGCTGCGAGGGTC |
| Internal bar code: | AAGCGGCAACTAGGGGGAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 98.2041 |
| LEAP-Seq n confirming: | 1203 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAGAGGACAGGAAGTGG |
| Suggested primer 2: | AAACTTGACTTGGTGGGTGC |