| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217914 |
| Chromosome: | chromosome 11 |
| Location: | 40319 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467527 | (1 of 1) PTHR24286:SF0 - CYTOCHROME P450 61 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACAGTCGCTTTCATAGCTGCCTGCTGA |
| Internal bar code: | GTCAGCTAGCCTGCTGGGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 99.5117 |
| LEAP-Seq n confirming: | 2853 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAAACGAAGTGTAGGGG |
| Suggested primer 2: | TACGGGTCCTTCACCATCTC |